Description
Plant Proteomics : Methods And Protocols, Hardcover by Thiellement, Herve (ED.... Plant Proteomics : Methods And Protocols, Hardcover by Thiellement, Herve (EDT); Zivy, Michel (EDT); Damerval, Catherine (EDT); Mechin, Valerie (EDT), ISBN 1588296350, ISBN-13 9781588296351, Like New Used, Free shipping in the US These 29 articles describe the most up-to-date methods of researchers in plant proteomics, concentrating not only on standard procedures such as two-dimensional gel electrophoresis but also on improvements such as immobilized pH gradients (IPG). Many of the papers cover tricky procedures and include phenol extraction of proteins from recalcitrant plant tissues, protein extraction from a variety of materials including woody plants, cereal seeds, xylem and phloem sap, isolation of chloroplast proteins or plant mitochondria, plant plasma membrane protein extraction and solubilization, detergents and chaotropes for protein solubilization, visible and florescent staining for two-dimensional gels, quantitative analysis of two-dimensional gels, protein identification using nano liquid chromotography and tandem mass spectrometry, identification of phosphorylated proteins, glycosylation, multivariate data analysis, electroelution of intact proteins from certain gels and their subsequent analysis, generation of plant protein microarrays and investigation of antigen-antibody interactions, and phosporylation studies using plant protein microarrays. Annotation ©2006 Book News, Inc., Portland, OR ()